Quality System and LOI Updates (August ’25)
LoI and QS Slides for NIH monthly mtg 28 Aug 2025
Letters of Intent Draft sent to C-Path on August 15
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On-line Resolution Calculator Applied to Etaluma Objectives
The following site may be useful to draw to people’s attention when specifying objectives on the Etaluma: https://www.microscope.healthcare.nikon.com/microtools/resolution-calculator/#Focus I have configured a custom resolution calculation using the sensor data supplied by Etaluma, here exemplified at 10x/0.3NA: To take account of the shorter tube lens (which gives ¼ magnification expected cf Nikon 200mm tube) I have…
Quality System Update (June ’25)
Status of the Quality System Team – June 27, 2025 Quality System Update – 27 June 2025
Macrophage Culture in the hToC Collagen I Gels Results in Decreased Macrophage Marker Expression
Introduction I recently established the feasibility of digesting cells out of the collagen I gels in the hToC bottom channels and reservoirs to use for flow cytometry. This involved using collagenase I and dispase II to digest fibroblasts and macrophages out of the collagen gel and running flow cytometry to evaluate macrophage marker expression. The…
A DDT for ICANS Risk Assessment in T Cell–Engaging Bispecific Antibody Immunotherapies: Strategy and Rationale
ByJimWork in progress … **************** T cell–Engaging Immunotherapies Pose a Predictable but Complex RiskT cell–engaging bispecific antibodies (BsAbs), particularly those targeting CD3 and a tumor-associated antigen, have emerged as a powerful class of immunotherapies. These agents leverage endogenous T cells to induce targeted tumor cell death, often achieving deep and durable responses in hematologic malignancies….
Modeling Basal Flow in Comsol
Introduction Our goal was to develop a COMSOL model for the bottom channel of the µSiM that can be accessed and easily used by anyone with a COMSOL license. Previously developed simulations for the µSiM rely on the CAD import module, which can introduce unnecessary complications with regards to accessibility and ease-of-use, particularly to expand…
The Yimei Method for Analyzing PDMS Beam Deflection in the μSiM Vasc-SkM
Image analysis for force from videos of deflected PDMS beams In the summer, Yimei Du from University of Rochester visited Truskey lab to help with the μSiM Vasc-SkM system. Besides working with the chip culture, she helped improve the image analysis of the videos of PDMS beam deflection for more accurate myobundle force analysis. We…