Gene vs Protein nomenclature

This is an update of the research on the on-chip EO pump. Due to a mistake in determining the direction of particle flow, it now looks like the particles in this post were moving by electrophoresis rather than electroosmosis.  The particles were negatively charged (-40 mV zeta potential) because of a COOH group on the…

Presentation in this post is based on the paper entitled “Large Area Synthesis of a Nanoporous Two-Dimensional Polymer at the Air/Water Interface”. Paper: Murray(King)JACS15 Supporting Information: Murray(King)JACS15 – SI Presentation Slides (PDF): Two-Dimensional Porous Membranes    

The goal of the design is to design a system that can co-culture cells under varying flow conditions, measure TEER, and perform dynamic microscopy. Specifically, the endothelial tight junctions are the primary mechanism by which the BBB is regulated. It is thought that astrocytes and pericytes can influence this mechanism. With the completed system, we…

Submitted to Acta Biomaterialia – March 15, 2014 Manuscript as PDF Check Status

Initial studies into the mechanical properties of pnc-Si were carried out using the “bulge test”.  With JP’s help, we’ve collected a large amount of pressure-displacement measurements.  This technique has been used to investigate thin film membranes suspended over windows of different geometries e.g. Vlassak.  In this study, we measure the deflection of a pressurized membrane…

Anant and I have been doing long-term discoloration experiments for the past few weeks.  We set up samples in “ideal” conditions – pnc-Si in transwells with pnc-Si side down, autoclaved samples and in the incubator (lower OH-).  The only non-ideal component is the use of DMEM+10%FBS with high NaHCO3 (3.7 mg/mL).  Chips were RTP’ed at…