Introduction I recently established the feasibility of digesting cells out of the collagen I gels in the hToC bottom channels and reservoirs to use for flow cytometry. This involved using collagenase I and dispase II to digest fibroblasts and macrophages out of the collagen gel and running flow cytometry to evaluate macrophage marker expression. The…
Introduction Over the past few months, I have been characterizing and comparing iPSC-derived monocytes (iMonocytes) with peripheral blood-derived (PB) monocytes. As I wrap up these experiments, we’ve decided to look at marker expression on iMonocytes and PB monocytes once they have been polarized to macrophages by culturing the iMonocytes or PB monocytes with HTEN051 fibroblasts…
Introduction Alzheimer’s disease (AD) is a neurodegenerative disorder that is characterized by accumulation of amyloid beta (Aβ) peptides and neurofibrillary tangles (NFTs) made of aggregates of the protein tau (1,2). It results in the destruction of neurons and their connections due to this accumulation of amyloid beta and tau (3). Tau is a microtubule associated…
