Quality System Update (June ’25)

The μSiM Vasc-SkM Microphysiological development can be separated into 4 parts: Media tuning for integrated culture Force sensing element setup Assembly of the μSiM Vasc-SkM and setting up the perfusion Transendothelial Electrical Resistance (TEER) measurement of the Vast component Work has been done on the first 2 parts. Media tuning for integrated culture In order…

CRE_Bispecific_Antibody_CRS_and_ICANS_Neurotoxicity_Guidance_Feb2024 51899285fnl_Immunotoxic Potential of PharmaceuticalsWe are considering Bispecific T cell engagers (BiTEs) [1] as an alternative to a CAR T driven cytokine release as an immunotherapy focused DDT. BiTEs are monoclonal antibodies engineered to have one arm that recognizes a T Cell surface antigen and another that recognizes a tumor antigen. There are number of…

Summary To improve the consistency of µSiM experiments involving circulating flow with peristaltic pumps, I designed a 3D-printable implement that securely holds small tubes in place. This simple design iterates on 3D printed tube holders or improvised tube holders that others have used previously. The purpose of this blog post is to share this tool…

Introduction I recently established the feasibility of digesting cells out of the collagen I gels in the hToC bottom channels and reservoirs to use for flow cytometry. This involved using collagenase I and dispase II to digest fibroblasts and macrophages out of the collagen gel and running flow cytometry to evaluate macrophage marker expression. The…

Work in progress … **************** T cell–Engaging Immunotherapies Pose a Predictable but Complex RiskT cell–engaging bispecific antibodies (BsAbs), particularly those targeting CD3 and a tumor-associated antigen, have emerged as a powerful class of immunotherapies. These agents leverage endogenous T cells to induce targeted tumor cell death, often achieving deep and durable responses in hematologic malignancies….