Nanopores May Eliminate Current Evaluation Processes Used for Detecting Epigenetics-Related Diseases

I had some old HUVEC (P8) on pnc-Si transwells and PET Costar transwells that I didn’t know what to do with.  So I tried a permeability study to see if there were any unexpected roadblocks in the protocol. I seeded HUVEC @ 50,000 cells/cm2 on PET and pnc-Si and allowed them to grow for 6…

Summary After success with the development and execution of the μSiM-CA (Canalicular Array; see Masters et al., Nanomedicine: Nanotechnology, Biology, and Medicine 2019), Jim wanted to develop a platform to answer some more biophysics focused questions. In order to facilitate these studies, the platform would ideally be configured to allow for live imaging of staph…

There is AFM images that were taken in Krauss’s lab before I left. I wasn’t able to see open pores. There were samples from two wafers (213 and 398) with deposited polymer. On samples from first wafer, the polyimide deposited very well and surface is smooth. On w398 the polyimide layer has hills which are…

Art is going to make a second stir cell this week. I asked him to replicate it as closely as possible, however I’m not sure how well that’s going to work. The one we currently have is made out of Ertalyt, a material similar to teflon. Paul gave us polyethylene which is much softer than…

For a while now I’ve been working on developing and testing the practicality of using nanoporous membranes as filters for highly sensitive assays. Traditional filter-based assays are restricted in their speed by both the time it takes for a particle to find a pore as well as the time it takes for the particle to…