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The following plot is an attempt to summarize recent work measuring rate of flow through a membrane as a function of concentration.  We investigated 100 nm NPs, (totally blocked), 20 nm NPs (partially blocked) and IgG (mostly passed).  In all cases, the buffer used was 1xPBS and 0.1% Tween 20.  This plot mostly shows the total…

Introduction We are aiming to make device assembly easy and quick, particularly for distribution to non-engineering laboratories. We are collaborating with ALine to develop assembly via pressure-sensitive adhesion techniques. Assembly Process Modular Device Assembly Animation2 We made ten devices with porous membranes and ten with non-porous membranes to show no leakage. Current Issues and Potential…

In yesterday’s post I showed that I was able to obtain good negative adsorption assay results, but there was one major problem. The cellulose and PES actually had higher protein concentrations after the assay. I suggested this was because they are drawing in water to some extent, but protein was not able to pass through…

I found another old wafer # 760 and asked Josh Miller to deposit a 25-50-25 OSO stack.  He did a standard RTP of 1050 C, 60 s, 100 C/s.  (and Josh Miller did the EDP etch, so this is really all his work) I measured a porosity ~4.5 %, and avg. dia. ~40 nm.  For…

Having finished the hydraulic permeability testing with DI water I wanted to see what the effect of an introduction of protein would have on the permeability values. To do this I simply reran a set of fresh membranes at 21.63psi using a 1mg/mL solution of bovine albumin in PBS instead of water.The group on the…

Tejas found this…  A cool article and video on water ‘sticking’ the surface. Notice the difference in advancing and receding angles. http://web.mit.edu/newsoffice/2013/droplet-surface-adhesion-0219.html