Utilization and development of in vitro µSiM platforms to study Bacterial Invasion of the Osteocyte Lacuno-Canalicular Network – MPS World Summit 2024 Poster

Previous posts: Theory Deposition Etching Imaging I have tinkered with the etch recipe some more, adding Argon to help prevent polymer formation (adding 5% O2 was not enough). The current recipe is 200 mTorr (100 mTorr Ar, 5 mTorr O2, 95 mTorr CHF3) at 120-125 W (6-12 W reflected). The plasma color is initially rosy (Argon), settling into a…

Manufacturer’s Data Logged by: Ahmet Gurcan Membrane Type: MSSN 4 micron Microslit SKU: MSSN400.C-3LX.1-4.0K2 Lot Number: 250409-10 Quantity Received: Notes: Manufacturer Test Report: Flow Data Avg in SLPM 2psi: 5.63 4psi: 8.1 15psi: 16.96 Burst: 37.35 PSI UR Testing Porosity: Pore size: Pore morphology: EDS: For more information visit:

Some background and a quick summation since I have done a great job at avoiding posting. My undergrad background before the lab Chemistry, physics, math (minor) and a random psychology minor biology I questionably knew what a cell was Overarching theme Putting a microarray on top of the membranes for cell culturing Currently cells are…

Introduction The on going work for the dialysis project at UR has undergone changes and many hours of trouble shooting over the past semester. We are at a point now that I would like to share all the work I have done up until this point including assay development, method progression, and the experiments we…

I’ve been culturing RPE cells over here in Rochester for my MgF2 fabrication paper, in which the goal is to make a permeable Raman compatible cell substrate. My previous work had shown polarization of RPE cells (via-ZO-1 staining) on nanoporous MgF2 nanomembranes, but in the meantime, I have reworked my MgF2 substrates to be thicker…

In prep for manuscript writing I came across a paper that says they’ve created a super efficient EO pump that uses extremely low voltages.  Their top flow rate (.125 mL/(min V cm2) ) is comparable to the flow rates I see with our materials (144 mL/(min V cm2)).  They run their experiments at 3 V…