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Urea Clearance: First Animal Study Results
BOTTOM LINE: We had great results from the small number of nanoporous membranes used to clear urea from Sprague-Dawley rats. We are clearing Urea better than commercial materials. (see fig) Recap of previous work: First we leaned how to set up the dialysis experiments using Sprague-Dawley rats. We made some rats uremic to elevate the…
Comparing growth rate of carbon on bare and alumina coated pnc-Si
This week, I did a systematic study on the growth rate of carbon on bare and aluminum oxide coated pnc-Si membranes. The bare samples were not treated prior to carbonization, while the alumina coated samples underwent a 5 nm treatment in the ALD tool. A bare and alumina coated sample were placed together inside the…
MgF2 grain size as a function of temperature
Summary Surface roughness is believed to impact the SERS effect. Rougher surfaces tend to produce more SERS activity. We can manipulate the npMgF2 surface by changing the deposition temperature, in the form of changing the grain nucleation, shifting the overall grain size and roughness. I performed TEM tomography and segmentation to pull out distributions of…
Applying a Voltage to Silver-Coated SNM Can Change the Rate of EO
Because I’ve been having such a difficult time measuring consistent zetapotentials (a big and heartbreakingly inconclusive post on those efforts is forthcoming) I decided to use one of my silver-coated SNMs as an EO pump and see if changing the voltage applied to the membrane changed the rate of EO. And I’m very happy to…
Badly Behaving BSA
For the charge model, we would like to know the surface charge density and size of our experimental molecules. I’ve attempted to use the Zetasizer for both measurements, but as mentioned in my diffusion paper, DLS gives sizes that deviate from the expected or crystal sizes. Since zeta potential measurements require large amounts of highly…
Cell Adhesion Testing 6/3-6/4
Two more adhesion tests were run recently, one on Tuesday and one on Wednesday. For both tests cells were imaged after five hours. Fibroblast trial 3 was the first test where the optimal cell density (assuming 100% of cells are detached by trypsin and 100% attach to the substrate) was measured using a hemacytometer. Of…