Many thanks to JK, Dean and Gunner (Webster HS) for compiling this table. 
Take homes …
We were worried that Friday’s experiments were giving us a false positive results. If the electrophoresis breaks the membrane, then we’d expect to see the rhodamine rush through just like it was in our experiments. It’s too hard to view the membranes afterward because they tend to break during removal. I designed some simple tests…
I am attempting to manufacture a range of npMgF2 thicknesses as demonstrated in my recent CCD post. Yesterday, I undershot my thicknesses substantially, I believe because of construction vibrations in the building. However, this results in some of the thinnest npMgF2 structure I have ever been able to image. I was targeting 15 nm, but…
Follow up to this post. As a brief reminder, I have performed 3-D simulations of separations in large and small volume systems. I have defined an effective diffusion coefficient that takes into account the membrane hindrance and compared this to free diffusion. As the Deff/D0 ratio drops, this means the membrane has a higher impact…
It’s been a while since I’ve done long-term BBB co-culture experiments on transwells but I recently completed a study. I was particularly interested to determine whether I could reproduce this spike in TEER that I measured in 1 previous experiment. I also needed repeats anyway. I followed the normal procedure – seeded P11 bEnd3 on…
Although the connection of those electrical circuits was always unstable, Greg and I did get some TEER measurement with this set up: Device A Day 1 Day 2 Day 3 Day 4 Day 5 EVOM 1 -3450Ω* -1000Ω -1200 Ω -1000 Ω / EVOM2 100 Ω -9600 Ω 450 Ω -3 Ω 827Ω Device…
Hi everyone, One of the objectives in the last aim of my PhD is to investigate the effect of soluble factors secreted by adipose derived stem cells (ADSCs) on wound recovery of fibroblasts (FBs). We have categorized these soluble factors to 3 subpopulations including: large-EVs, small-EVs, and non-EV associated molecules. For the last 3 weeks,…
