Similar Posts
AFM Training
Yesterday, Chris, Dave, Graham and I were (exhaustively) trained on the Asylum AFM in Rick Waugh’s lab. We had an overview of the basic features of the AFM and got some real data, as well. The top panel is a height image of the pnc-Si chip with the well side down. Notice the slight decrease…
Track etched nanoparticle permeability
I used the transwell track etched membranes for these permeability experiments. I first looked at 5nm gold in 10mM KCl. After 24 hours the retentate and filtrate had just about equilibrated: We can compare these scans to the stock 5nm stock solution and see that the height of the 525nm peak is related to the…
Plate geometry matters in Tecan!
Christine and I have been using the present Corning 24-well plate definition in the Tecan for our experiments so far. Yesterday I placed a pnc-Si chip in the (approximate) middle on the bottom of 1 of the 24-wells. I did a 500nm absorbance scan in a circular pattern (15×15 points) – to get data similar…
PEGDA Microarray and PEGDA reactivity issue
My goal and focus of the last post is to to use the existing SU-8 grid structure used on the ultrathin silicon oxide lift off membranes to create . To accomplish the goal the SU-8 surface needs to be modied to decrease cell adhesion so that the cells are trapped in the wells. Please see…
Updated Burst Pressures Picture
I only added coordinates on each data point.
Adenovirus Captured and Imaged
Last week we were able to capture and image individual virions on the nano membrane without a PETL using a simple drop-on-membrane protocol to establish some pull through. This was done to to verify that the individual virions will actually be caught in the pores and that our EM preparation protocol was efficient for drying…