TEER of 1st 2 cocultures
My previous 2 posts on co-cultures presented images but I also tracked the TEER in these experiments. This post shows that data.
The top 2 graphs are from the 1st co-culture experiment – the bottom graph is from the second experiment (where my PET controls died), as % Day 0:
On PET, the coculture TEER was higher than bEnd3 alone and the NG108-15 cells didn’t contribute to the overall TEER. On pnc-Si, NG10815 cells increased the TEER after ~ 7 days – this is because the cells were clumping in the wells of pnc-Si. The co-culture followed the bEnd3 TEER except for day 7. The % increase on pnc-Si was less than that on PET. Interestingly, the second experiment showed a steady increase of TEER for the co-culture and plateau for bEnd3 only on pnc-Si (like in experiment 1). I had to stop this experiment at day 7. In these experiments, I noticed that the NG10815 media became considerably acidified over time. It’s possible that this acidic environment adversely affected bEnd3 cells, thus inhibiting maintenance of barrier function in the coculture at late times.
Unfortunately, the PET, but not the pnc-Si, samples exhibited the expected trend. However, it’s possible that the day 4-10 timeframe is the ideal window for barrier function experiments.
UPDATE(MAY 17, 2010): TEER Data as “Net TEER”:
