NSF STTR Project Review Session 1

I guess we weren’t sure if I was actually drawing rhodamine back through the membrane or just bleaching it. So I made a new apparatus which consisted of a sawed off sepcon mounted on a slide with channels etched into the lower plastic of the device to connect a pbs resevoir on the slide to…

What happens to cells when they are exposed to a series of bubbles with and without a dubbler? The purpose of this experiment was to prove the hypothesis that bubbles damage cells, and the dubbler prevents cells from experiencing bubble damage. The experiment was performed with a PBS flow rate of about 0.14 mL/min through…

In order to figure out the relationship between grain size and pore size, I tried to use XRD measurement to determine the grain size by Scherrer equation. t = kλ/(B cosθ) where t is the average grain size, k is the shape factor that is in the range 0.87-1.0 (it is usually assumed to be…

From my last two posts (Anodic Bonding, Ozone Bonding), it is clear that we have some sort of bonding going on between the freestanding membrane and the posts intended to support the membrane. However, we do not understand the nature of the transfer. To gain more insight into this process, I took more SEMs of…

Introduction While we have optimized coculture models for commercial brain endothelial cells and pericytes, we needed to do the same for iPSC-derived cocultures. In this post, I show the optimization process using IMR90-4-derived EECM-BMEC-like cells [1] and brain pericyte-like cells (BPLC) [2]. Methods The top well chip surface was coated with collagen iv (Sigma C5533), bovine fibronectin…