Question about observation through the plastic substrate of the new diffusion chamber
Attached are the photos taken under DIC and Rhodamine channels, using the new diffusion chamber.
I can clearly observe Rhodamine pass to the lower hole of the chamber, under the Rhodamine channel by eye ball. But from what I got using the Matlab software, it looks weird because both DIC and Rhodamine photos look very similar.
I will definitely try it again but what do you guys think about this observation? Thanks!
Sorry missed the titles… The first image is Rhodamine and the second one is DIC, very similar though…
Lingyun, it looks like both images are from the Rhodamine channel. I’m not sure how you’re getting your images, but if you export them the rhodamine file will be appended with an r and the dic with a d at the end of the file name. I can help you with the matlab program too if you need a hand.
The other thing I’d like to mention is that it’d be nice if you could submit a movie with your results. It’s hard for us to tell if the rhodamine is going through or if it’s just the way you’ve thresholded the image. Also it’d let us know something about the rate of dye passage. I can help show you how to compile time course movies into gif images for the web too.
How long did you let the rhodamine sit before taking the pictures? Is the gain adjusted correctly? I don’t think dye is passing through based on these pictures…
I didn’t use a good membrane since I just wanted to test if I can see rhodamine through the plastic substrate. I am sure the lower hole was filled with rhodamine because I could see very strong rhodamine signal with eye ball.
– Jess, I didn’t mess up different type of images. If you leave your mouse over the images, you can see the file name at the bottom line of firefox. They are named 2_r_1 and 1_d_1, respectively.
-Dave, I think both images look like DIC channel. That’s different with what I saw with my eye — this is why I said it was weird.
Ok, I will do a movie tomorrow.
I guess its possible that a saturated DIC image and a rhodamine image could look very similar. Lowing the overhead lamp should make it clear that these are different modes. Jess and I are gone today. Please grab Henry – 347-583-4171 to make sure your images are optimized.