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f-BSA Adsorption on Adarza functionalized pnc-Si chips
Chips of pnc-Si were funtionalized at Adarza. They returned four groups of chips. Ethanolamine, Amino PEG-1, Inactivated sLinker, and Amino PEG-2. These were all incubated with PBS and 5-mg/mL f-BSA (2:3) in a humid refrigerated dish. The samples were rinsed in PBS then DI water and dried with filtered compressed air. The measurements were taken…
Makerbot Replicator 2x Open for ABS prints
Recently, Jim has purchased a Makerbot 2x printer for our group. I’ve spent the last few weeks printing and debugging. It has the capability to print many different types of material, along with 2 separate nozzles. I have been able to get many fine prints done with ABS plastic, and I’ve put together a basic…
PSi and pnc-Si degradation, again
I repeated the first degradation experiment (posted here) to confirm the results. I also tracked discoloration of the samples this time. As before, I used 1X PBS, but this time, the entire assay was performed in the 37C oven. Discoloration pictures: The PSi sample starts with a new rainbow coloration and then completely discolors to…
Cells Growth in Different Media
ByAslanThe goal of this set of experiments was to study the growth rate of cells in 3 different media; 1. Complete Media 2. Exosome Free Media 3. Serum Free Media. We studied the effect of media on both ADSCs and FBs. Cells growth was monitored by a Matlab code that Henry has made for us….
Effect of salt on pores (benchtop)
(Transferred from old blog) The above TEM images show that RTP (800C, 5 min) treated samples (from W334) have their pores intact in salt over a period of time! 1M and 2M NaCl solutions were used and TEM samples were taken at different time points. There is some deformation observed near the edges of the…
Thickness and pore-size selectivity of neutrophil transmigration: Which membrane parameters matter and how would they affect transmigration?
Continuing the studies on neutrophil transmigration in devices with embedded parylene membranes of different pore sizes and thicknesses, three pore sizes (1.0 um, 1.4 um and 1.7 um) and two thicknesses (0.7 um and 1.2 um) were used for these sets of experiments. Thickness ratio of 1.2 um to 0.7 um roughly correlated with…