Counterflow Dialysis

After correcting a minor error in my code that was giving me some weird limiting behavior, and optimizing the code to give me faster results, I generated the following curves: Note that, as experiment predicts, the ‘sharpness’ of the graph increases with increasing applied pressure. The model isn’t exact, and slightly underestimates the actual sieving…

Adding pockets (250 micron long, 76 micron deep) to the bottom of the channel (channel is 500 microns wide, 125 deep) in a 4 layer PETL  (1 inch long chip) promotes disruption of laminar flow.

This post presents the data from all of my TEER experiments of BBB co-cultures, with bEnd3 endothelial cells on the bottom and NG101815 glia on the top of PET and pnc-Si transwells.  For all experiments, I used 50000 cells/cm2 as the seeding density.  I always had 3 samples – bEnd3 alone, NG10815 alone and the…

Introduction The invasion of Staphlyococcus aureus into the cracks of cortical bone leads to osteomyelitis, a devastating disease often resolved by amputation [1]. The μSiM platform has been used to study the genetic mechanisms of S. aureus invasion. The membrane pores mimic the network of cracks in bone, referred to as the osteocyte lacuno-canalicular network…

I posted some pictures yesterday from AFM last week.  I got back onto the AFM today and re-scanned the megasonics cleaned virginia semiconductor wafers.  For each image, the top and bottom panels are the height and ZSensor scans, respectively.  All scans are 10um x 10um.  I did a linear analysis of the ZSensor data for…