PETL mixer

In this post we look at the effect of altering the area available for filtration on a standard 5 slot SepCon.  Karl and I did this by simply cutting gaskets such that they covered different regions of the surface of a chip.  We have three configurations, 1, 3 and 5 slots.  Here’s a picture of…

Cancer cell metastasis is responsible for 80 percent of cancer-related death. The metastatic cells migrate through tight constricting spaces, therefore constricted migration and its enabling mechanisms have received a lot of attentions in recent years. The most famous research groups that are working in this field are Lammerding group, Discher group, Wolf and Friedl group,…

Hey all, I have the first draft of my ASEMV poster here for any feedback that you might have. The conference is in Monterey, CA next week and any feedback that you have on this by Tuesday or Wednesday evening of this week on the poster would be awesome. ASEMV_Poster_KL_09132017_01  

Introduction This post highlights results from my second attempt to differentiate hiPSCs into endothelial progenitor cells  EECM-BMEC-like cells in our lab. For details on methodology, see Nishihara et al., FASEB, 2020 and my previous post. Methods Cells were seeded at 70,000 and 100,000 cells/well on D-3. Only the 100,000 cell/well plate was sorted. Due to media being backordered,…

This post is a continuation of the affinity based capture of streptavidin conjugated EVs. The difference is that instead of using the tangential flow system we used the ALine devices. Sam’s previous work discussed the use of ALine devices for size based capture of EVs, but in this case we wanted to try the streptavidin-biotin…

For this experiment, I looked into the stability of RTP treatment.  Specifically, I tested discoloration rates for chips at different time points after I did RTP.  The idea was to see if the beneficial effects of RTP depended on the time that chips were stored after RTP processing.  Here’s the data: All chips were from…