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Cancer Cell Migration and Wound Healing
A few weeks ago, Henry and I ran a migration/wound healing experiment using two cancer cell types: T24 (bladder cancer) and MDA-MB-231 (breast cancer). MDA cells are highly migratory, while T24 cells are less so. We wanted to test if the exosomes from MDA cells would cause T24 cells to become more migratory. This was…
Protected: SERS Measurements on 150C, 250C, 350C 1236 npMgF2
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Cell counts on free-standing vs. supported pnc-Si
In most of the images I’ve posted of bEnd3 on pnc-Si, it seemed like there were more cells crowded onto the free-standing pnc-Si compared to supported pnc-Si. This post shows that this is indeed true. To do cell counts, I stained cells with Hoechst 33342 (1uM in media) for 15 minutes @ room temperature and…
BBB Device V2.3
I’ve made some modifications to my V2 design such that it allows total vertical access, among other things: This design utilizes embedded electrodes and filter within gasket layers to create a tight fluidic seal for each channel. Here, the slot is pointed upward, so the endothelials will be on the bottom side and the…
pH Variation of DMEM
(Transferred from old blog) Aim: Determine how quickly DMEM pH changes once removed from the incubator Methods and Materials: • non-sterile complete Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% FBS • 6 and 24 well cell culture plates • 37 ºC 5% CO2 incubator 2 mL and 5 mL DMEM were placed in a…
ZO-1 Staining on ARPE-19 Cells grown on MgF2 nanomembranes
The following images are from ARPE-19 Cells cultured for 3 Weeks over here in Nottingham (thanks for help from Emilia Moradi, Victoria Ciampani, and Kevin Webb). MgF2 nanomembrane chips were positioned into a transwell insert and sealed using PDMS, a biocompatible polymer, creating a two-compartment transwell. After an ethanol sterilization, ARPE-19 cells were seeded in a 12 well…