Counterflow Dialysis

Karl and attempted to pass 10, 20, 40 and 50 nm Au NPs through samples from the center of wafer # 1019 (avg por dia 50-60 nm, porosity ~27%).  The bottles of stock Au from BBI solutions do not have the concentrations marked, but according to BBI’s website, they only sell the concentrations provided in…

Previously, I had data showing that 5nm gold in 10mM salts went diffused through pnc-Si membranes.  I was able to achieve this twice, although the second time took two days before the gold signal was intense enough to observe via absorption. Here’s what has happened recently: 5nm gold will not pass through untreated SC 168,…

AFM images report Protocol: pnc-Si TEM  grids were mounted on thin silicone inserts. Surface treatments were applied to the grids. Except for the CellTak treatment, the treatments were all diluted in Hank’s Balanced Salt Solution (HBSS) and incubated with grids  for 2 hours and then washed off with PBS. CellTak was diluted in 0.1 M…

Introduction Previous experiments completed by Hayley Miller investigated the effect of small extracellular vesicles (EVs) collected from Adipose Derived Stem Cells (ADSCs) on wound closure velocity and cell speed. Her previous related post can be found here.  It was initially hypothesized that EVs play an important role in wound healing and affect the wound closure…

The Tecan Nanoquant plate is amazing.  For those of you who are unaware, we were able to demo a quartz plate that enabled absorbance measurements with only 2ul drops.  It took a little bit for me to figure it out, but it really is a great tool for this kind of study. The ultra low…

Last week, I deposited silicon nitride/ amorphous silicon/ silicon nitride stack and then annealed the free standing stack after etching the substrate. The nitride film was deposited with 25W bias and the silicon film with 5W bias. The thickness for the nitride film is 30nm where it is 25nm for silicon.  One of the initial…