AFM Scans of Coated, Annealed TEM Windows

AFM images report

Protocol:

pnc-Si TEM  grids were mounted on thin silicone inserts.

Surface treatments were applied to the grids.

Except for the CellTak treatment, the treatments were all diluted in Hank’s Balanced Salt Solution (HBSS) and incubated with grids  for 2 hours and then washed off with PBS. CellTak was diluted in 0.1 M sodium bicarbonate, pH 8.0 for 20 min and then washed off with water.

Scans were taken of the filter and the bulk silicon regions using AFM.

This week we are culturing ARPE-19 Cells (retinal pigment Epithelium) on the membranes. Hopefully they will stick nicely.

Roguish Adventures!

 

Supposedly a gang of outlaws in Sherwood Forest met under a rather large oak tree....All the joists keep it from splitting. It's quite impressive.
Supposedly a gang of outlaws in Sherwood Forest met under a rather large oak tree….All the joists keep it from splitting. It’s quite impressive.
Charge! Wouldn't it be cool to own an awesome topiary?
Charge! Wouldn’t it be cool to own an awesome topiary?
Robin Hood! Outside the castle walls. His merry band of miscreants are off in the background dozing.
Robin Hood! Outside the castle walls. His merry band of miscreants are off in the background dozing.
Duchal Estate on the ruins of Nottingham Castle.
Duchal Estate on the ruins of Nottingham Castle.

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