AFM Scans of Coated, Annealed TEM Windows
Protocol:
pnc-Si TEM grids were mounted on thin silicone inserts.
Surface treatments were applied to the grids.
Except for the CellTak treatment, the treatments were all diluted in Hank’s Balanced Salt Solution (HBSS) and incubated with grids for 2 hours and then washed off with PBS. CellTak was diluted in 0.1 M sodium bicarbonate, pH 8.0 for 20 min and then washed off with water.
Scans were taken of the filter and the bulk silicon regions using AFM.
This week we are culturing ARPE-19 Cells (retinal pigment Epithelium) on the membranes. Hopefully they will stick nicely.
Roguish Adventures!



