Tendon-on-Chip Platform for Modeling Inflammation and Fibrosis in Tendon Injury
This is my draft presentation for my CMSR presentation on December 4th.
This is my draft presentation for my CMSR presentation on December 4th.
The new sputtering system is scheduled for delivery at the end of June. It will bring several advantages over our current system; which translates (hopefully) into stronger and more consistent material. 75 days… AJA Sputter Full Blueprint
Background: The human Tendon-on-Chip (hToC) spans collaboration between the Center for Musculoskeletal Research (CMSR) at the UR and the McGrath lab to develop an in vitro platform to study inflammation, fibrosis, and cell-cycle regulation in fibrovascular tendon healing. Interaction between the blood-tendon-barrier (BTB) is fundamental to the investigation of this pathology as part of the…
Following images show the diffusion profile of rhodamine across pncSi as observed in the timelapse movies. Looking at the profiles no significant difference is observed between the non RTPed and RTPed samples. Non RTP sample: RTP sample:
I have just completed testing and documentation of a MATLAB GUI designed to allow anyone to make predictions of the clearance behavior of solutes though our membranes in tangential flow. I’m uploading the file here, which includes all the MATLAB code for the GUI, dependancies, and documentation. It will also appear under the resources tab on the…
This experiment was carried out to determine the maximum life of pnc-Si in cell culture conditions based on discoloration. All the discoloration slowing factors were put together to see how long would the membranes last for. These include: samples in 3D format (Sepcon) with ‘membrane side’ down (with no intact membranes), all in incubator, DMEM…
AFM images report Protocol: pnc-Si TEM grids were mounted on thin silicone inserts. Surface treatments were applied to the grids. Except for the CellTak treatment, the treatments were all diluted in Hank’s Balanced Salt Solution (HBSS) and incubated with grids for 2 hours and then washed off with PBS. CellTak was diluted in 0.1 M…