New sputtering system

As part of my work over at the University of Nottingham, I’ve been using iBidi devices to grow cells. Eventually, I will use their product to do Raman studies of cells grown on my MgF2 substrates. I’ve been making some progress towards that end using PDMS (I’ve been using 10:1 base to curing agent for…

Attached is a paper I’d like to review on Thursday (June 11, 2015). This group is using two planar surfaces in an attempt to mimic a 3D cell culture environment. This is similar to our claims that cells growing on a permeable membrane can be akin to a slice of a 3D matrix. I met…

This post is meant to conclude the work that I have completed this summer with the double slot single pass devices; recently I have conducted additional experiments and collected ultrafiltration data. In my previous post, “Single Slot Inverted-Chip and Double Slot: Single-Pass Dialysis Devices” https://trace-bmps.org/single-slot-inverted-chip-and-double-slot-single-pass-dialysis-devices/ I was exploring how various analyte / dialysate flow rate…

My goal and focus of the last post is to to use the existing SU-8 grid structure used on the ultrathin silicon oxide lift off membranes to create . To accomplish the goal the SU-8 surface needs to be modied to decrease cell adhesion so that the cells are trapped in the wells. Please see…

I went to Kidney Week in Philly, Nov 13-15. American Society of Nephrology. I spent most of my time in the Bioengineering talks and posters. Here are some highlights. I put the Abstracts pdf on the cloud. Recellularization of Vascular and Tubular Compartments of Rat Kidney Scaffolds with Embryonic Stem Cells   Basically, they perfused…

All of my recent posts on VEGF, VEGFR2 and NF-H imunofluorescence have included data from cells grown on coverslips only.  This was my first attempt to try to do immunofluorescence staining on intact pnc-Si transwells and then pop the chips out of the transwell plastic in order to image at 100X.  Since VEGF is involved…