Following images show the diffusion profile of rhodamine across pncSi as observed in the timelapse movies.
Looking at the profiles no significant difference is observed between the non RTPed and RTPed samples.
Non RTP sample:
RTP sample:
Introduction Sphingosine-1-phosphate (S1P) is a bioactive sphingolipid with known paradoxical effects on endothelial cell (EC) barrier regulation. S1P binds 5 GPCRs: S1PR1 (Endothelial differentiation gene (EDG)-1), S1PR2 (EDG-5), S1PR3 (EDG-3), S1PR4 (EDG-6), and S1PR5 (EDG-8). Despite the aliases, endothelial cells predominantly express S1PR1&3. S1PR1 has been shown to have barrier protective or enhancing effects on…
In 5.4 mm single slot chips, sheep blood was flowed at 3 µL/min though the 3 mm x 1 mm channels with PBS flowed at the same rate but in the opposite direction for four hours. The chips were pre-wetted with PBS and rinsed following flow. The membrane surfaces were fixed with 4% paraformaldehyde for…
Today I looked at 3 more wafers: w158 (9/7/07) – some pinholes, overwhelming amount of very fine debris, not cbs as far as I can tellw160 (9/7/07) – some pinholes, debris, no cbsw171 (9/26/07) – many pinholes, lots of debris, no cbs w158 (200x) – small particles Link to updated report.
Discoloration Assays Introduction: pnc-Si chips were monitored for discoloration to find chips that had not been previously RTP’d. These assays were necessary in order to find chips that could potentially be used for cell proliferation assays later. Setup: pnc-Si chips were autoclaved and placed in a 24-welled plate with 300uL of DMEM and incubated at…
As I mentioned in my last post, I will be performing protein diffusion tests through CytoVu assemblies (from one well to the other) and measuring the amount of protein that is still in solution when the system reaches equilibrium in order to determine the amount of protein that is adsorbed to the assembly and thus…
This is the second in my series of posts that will be on my model exosome system. As I am trying to define the appropriate conditions for separating exosomes from blood and other fluids, I am first going through and trying to isolate each important parameter and see how they affect the capture of these…
