Journal club

I had some old carbonized samples kicking around and decided to do some high res TEM yesterday to see what a completely occluded pore looked like.  Below is an image showing a blocked pore and adjacent Si nanocrystal. Inside the pore, you can see concentric “rings” of carbon atoms i.e. graphene sheets?  Here is a…

One of the other suggestions from Angela Glading at lab meeting a while ago was to quantify the vacuoles on different culture areas.  So, I selected images from 3 different experiments (all at day14 of growth) and counted the number of cells which expressed vacuoles on different areas.  I then used my mask code in…

In the wake of the apparent loss of all of the phosphatase in a sample that was placed into a CytoVu well, left for 24 hours, and then checked for phosphatase activity, it’s become clear that the adsorptive qualities of the CytoVu assemblies needs further characterization. I showed in an earlier post that there was some loss…

As many of you know, we have been having a lot of trouble with the new etch cell and have not been able to produce high quality membranes. Yesterday, we discovered that the stainless steel used to fabricate the new cell is different than that of the original cell. Here are the EDAX spectra: Old…

When Aslan and I went to the Beckham lab last week, they gave us a copy of their ultracentrifugation protocol for isolating exosomes. I attached it here so that we can have it as a reference. The major thing to note is that they typically use 13-15 mL volumes of media to begin their centrifugation…