Manufacturer’s Data
We used chips from the latest 5 slot SepCon NPN wafer 1142 (14%, 36nm) to test our ability to separate 20 nm “blue fluorescent” polystyrene NPs from Invitrogen from IgG. We used a concentration of 10^14 particles/ml NPs and 1 mg/ml IgG. (This is a mixture of fluorescent and unlabeled IgG) We compared forward vs…
In an effort to conserve membrane samples I am working to run diffusion experiments with a mixture of BSA and DDM. This will only use 2 chips instead of 4. In our meeting on Tuesday it was mentioned that BSA can be detected using the TECAN at 280nm, while DDM is not detected at that…
For this experiment, 3um diameter pores were created using SimPore’s mask. Reactive Ion Etching was used to form the pores in the SiN. Both 25nm and 150nm TEOS oxides were used. An SU8 Structure of 10um lines with 100um square windows was chosen. A sample was placed in 10:1 BOE w/ surfactant for various amounts…
In our study of endothelial fibronectin fibrillogenesis and FA formation on microporous SiO2 membranes, we decided to investigate whether the pore orientation and pore spacing induced FA formation and therefore F-actin alignment. (Note: we updated our last post on FN fibrillogenesis here.) Spencer and I have been busy analyzing data and making figures. It has…
SU-8 3010 has been used to create a scaffold in the fabrication of the liftoff membranes. The supplier of the negative resist, MicroChem, rates it for a 13 moth shelf life. We were having difficulty achieving good liftoff with membranes in the second half of last year. The SU-8 had expired by several months, so…
The permeability data is very different for films deposited at room temeperature and 400C.The 400C deposited wafers have much higher permeability and more consistent. w335 – RT w336-RT w338 -400C w340 – 400C Burst Pressures
