Endothelial Vacuolization Induced By Highly-permeable Silicon Membranes

I finished 3 independent experiments of air-blood barrier co-cultures – these consist of bEnd3 endothelial cells on 1 side and RLE-6TN lung epithelial cells on the opposite side of the membranes.  We didn’t expect these cell types to potentiate TEER in anyway (like we expected glial cells to tighten endothelial barrier function).  However, our collaborators…

Recently, I have been trying to pass 5 nm, 10 nm, and 15 nm gold through untreated, carbonized, and ozone/carbonized membranes.  These experiments were done in the pressure cell with fresh samples.  I used a 1:1 dilution of Au stock and DI water.  To wet the membrane I placed a 40 uL drop of water…

In continuation of the previous post, its been further observed that O2 RTP treated samples remain unchanged whereas the Ar RTP treated samples started to discolor after a week, as shown in the following images: In order (left to right)1. Ozone+Ar RTP2. Ozone+O2 RTP3. Control (Untreated)4. Ar RTP5. O2 RTP

Introduction The work in this post explores the hypothesis that durotaxis affects the invasion of S. aureus into the osteocyte lacuna-canicular network (OLCN). To address this hypothesis, we modified the µSiM to enable the independent tuning of pore stiffness, ranging from those of soft-tissues to that of silicon nitride (~10X stiffer than bone). More background…

I know this is my third post today, but this is not a data post. It’s more of a PSA. If you want to add beautiful equations into your post (like I did here) then rather than generating a bunch of images and inserting them into the post, you can simply use . is the…

Let’s get a blast-from-the-past and go through a bunch of drawers in the lab!  I spoke with Jim and it seems like it might be best to schedule it during next regularly slotted Tuesday NRG mtg.  So let’s plan on meeting downstairs in the lab on 2-19 at 9:45 am. Also, I bought an assortment…