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BBB co-culture (2)
In my last post (the first co-culture), I showed that bEnd3 cells exhibited a different morphology when they were co-cultured. I also decided that I would reverse the orientation of the co-culture in order to better visualize the endothelial barrier. That is, grow bEnd3 on the basolateral side and NG10815 on the apical side of…
Wound Maker
The purpose of this device is to make uniform and straight scratch wounds in a 96 and 24 well plate for migration experiments. Previously, scratch wounds were made by hand using a p-10 pipette tip by scraping the tip in a straight line along the bottom of the well. Not only is manually scratching the…
SiO2 coated membranes
The SiO2 coated samples (from W335 & W341) were found completely discolored after 5 days in DMEM (benchtop). Thus, silicon dioxide coating didn’t prevent discoloration as much as the RTP treatment. There’s a need to check the discoloration profile of the SiO2 coated samples within 5 days. Till 24 hrs. there was no discoloration observed….
Creating Unwanted Vacuoles in Endothelial Cells With Flow – Mossu et al – unpublished
ByBrittaVacuoles with pulling flow vs pushing flow This video shows an example of the first trials with the uSim flow cell (Tejas’ early versions) when creating laminar flow over a monolayer of BLECs – see Mossu et al, JCBFM 2018 – the issue we had to overcome was to avoid creating vacuoles in the endothelial…
Retest: SC256 Diffusion with a Mixture of BSA and DDM
ByMike HOn Friday I posted results from a diffusion experiment in which I tested “treated” and “untreated” SC256 samples against the diffusion of a mixture of DDM and BSA. The DDM-BSA solution contained an initial DDM concentration of 1.0% and an initial BSA concentration of 2.5mg/mL; the solution also contained 20mM HEPES and was at a…
PDMS-filled wells don't increase sensitivity for HMM detection
This post is related to the HIV drug screening project that Carrie has been working on. To refresh everyone’s memory, the aim of this project is to use pnc-Si as a filter to separate high molecular mass (HMM) protein-RNA complexes from smaller protein monomers/multimers. Small molecules that break down the HMM form of this protein…