PhD Committee Meeting and Internship with Sartorius

A couple weeks ago when I presented at lab meeting, Angela Glading suggested that I look at epithelial cell vacuolization on transwells.  This was in order to determine if vacuole formation was endothelial cell-specific.  If I’m remembering correctly, the rationale was that the vacuole-less fibroblasts I showed were not contact-inhibited cells and thus not a…

So as most of you know by now, I have been working on developing microfluidic systems to study the leukocyte-endothelial interactions in vitro. I have developed systems that can facilitate the basic biological assays involving neutrophil migration and live-imaging. In this blog, I am presenting the latest (and the last) results on the effects of…

Very last minute but here goes … Voting closes at 7 pm tonight. Vote for 2! [yop_poll id=”4″]  

I tested all the microbeads that the Gaborski and Lapizco-Encinas labs own.  The results are summarized below.  The sizes ranged from 50 nm to 2 µm.  I mixed them to concentrations of 0.2 g/L for all bead less than 1 µm and 1.0 g/L for all 1 µm or greater.  Interestingly, the scans look rather…

Over the past week Andrea and I designed a new co-culture support structure for use with SiMPore’s 5.5 mm square membranes. The devices are built for use in standard 24-well plates and should prove useful to anyone wanting to do basic co-culture with these membranes going forward. The device is constructed of PDMS rings as…