As a continuation to my previous post, I am running a triplicate of cell culture adhesion tests to observe cell lose on our membranes following physiological shear stress.
HUVECs (P3,4,5) were seed within our mimetic and left to grow to confluent for 24 hours. Shear (4.5 dyn/cm2) was initiated and images were collected following 5 hours.
Some of my recent cell culture work on Microporous MgF2 windows have shown vacancies in the cultured monolayer after fixation and staining. We should see a contiguous layer. To better understand my fixation, I seeded and cultured P10 RPE-19 cells at a high density on a coverglass and in a polystyrene dish for a week….
The unwashed membrane has a definite “film” over it which makes the pores look textured. The MeOH & H2O seems to clear the pores up, but there are some places where there is remaining PVP that sticks to the inside walls of the pore.
Hello everyone, presented here is a theoretical perspective on the shear stress reduction enabled by our shear-free microfluidic system. Briefly speaking, the shear-free microfluidic system consists of three main components: a flow compartment, the pnc-Si membrane, and a shear-free compartment. The membrane attenuates the fluid flow from the flow compartment to the shear-free compartment while permits…
This data comes from our collaborators in Switzerland who are working on a BBB culture model using NPN. In this study they are looking at the effects of co-culture on barrier function. The co-culture is with pericytes and a vascular brain endothelial cell line that they are cultivating. The permeability is measured with the passage…
I’ve upgraded the WordPress publisher to version 2.6. The most useful change is the ability to track changes to any post/page, compare revisions, and revert to an older version. Here’s a video showing the changes: https://videopress.com/v/mARhRBcT/fmt_std Additionally, I’ve installed some plug-ins that should make posting and keeping up with new posts easier: There is now…
Kevin was kind enough to share his presentation with us from his visit this July: Here is the link on Owncloud: http://drive.bbe.23a.myftpupload.com/public.php?service=files&t=c3eceae62439a478b4d1b676924e45ee
