The unwashed membrane has a definite “film” over it which makes the pores look textured. The MeOH & H2O seems to clear the pores up, but there are some places where there is remaining PVP that sticks to the inside walls of the pore.
I finished the first experiment to look at rhodamine transport across commercial transwell membranes. I was mainly interested if there would be enough transport into the basolateral chamber to measure the rhodamine with the spec. Experimental details: both chambers in PBS and at room temperature, spinning on the rotomix, 0.5mM initial rhodamine in donor; sample…
At Nanotech I met a professor from Geneseo, K. Yokoyama, who worked with gold in sol-gels. He found he was able to dissolved gold in sodium tetra bromate (Na2Br4O7) without it aggregating. I have attempted to dissolve gold in other salts to no avail. This is an important step in testing membrane separations with gold,…
In our study of endothelial fibronectin fibrillogenesis and FA formation on microporous SiO2 membranes, we decided to investigate whether the pore orientation and pore spacing induced FA formation and therefore F-actin alignment. (Note: we updated our last post on FN fibrillogenesis here.) Spencer and I have been busy analyzing data and making figures. It has…
Discoloration Assays Introduction: pnc-Si chips were monitored for discoloration to find chips that had not been previously RTP’d. These assays were necessary in order to find chips that could potentially be used for cell proliferation assays later. Setup: pnc-Si chips were autoclaved and placed in a 24-welled plate with 300uL of DMEM and incubated at…
About a month ago, I posted some preliminary data that showed NaCl diffusion through SC348. This was motivated by my observation of vacuole formation on SC348. Since my hypothesis is that cell culture substrate permeability is the key factor for vacuole formation, cells shouldn’t have formed vacuoles on SC348. Unless it is actually porous. This…
[yop_poll id=”3″]
The brightness of the image may even be significant. Was the beam noticeably dimmer with the thick PVP coating. IF so, the film is very thick.
Anyway, the pores are clearly covered, so the fact that water passes so easily must mean that the film either dissolves or is highly porous. It will be interesting to see if there is an effect on separations.