ICNMM Presentation

On Friday I posted results from a diffusion experiment in which I tested “treated” and “untreated” SC256 samples against the diffusion of a mixture of DDM and BSA. The DDM-BSA solution contained an initial DDM concentration of 1.0% and an initial BSA concentration of 2.5mg/mL; the solution also contained 20mM HEPES and was at a…

From my last blog post, was talked about designing an assay whereby the NPN filter would exclude DNA-conjugated gold nanoparticles while allowing short DNA to pass. Since then, I (with the help of my colleague Dan Tassier) have been wrestling with gold nanoparticles to find conditions in which the nanoparticles were stable, while also allowing…

In this previous post (also some in this post), I had displayed the difference between the 120nm SiO2 microporous membranes and four different iterations of Track-Etched insert membranes in reference to nuclear (DAPI, blue) and actin (Phalloidin, green) stain. I recently performed the same staining protocol on other substrates: High Pore Density Microporous 300nm SiO2, Low…

Here is a better, more thoughtful, attempt at pore processing of a single image of the backside of a 75-nm thick membrane (four slot). One thing you will note that could be different from the analysis of the frontside of the membranes: The small openings that appear quite sharply defined here could be missing from the frontside…

The competition keeps coming at us  … Liu(Cui)JVSTB12

Wafer 302 appears to have no pinholes and held up pretty well in the centrifuge, up to 5psi for many hours in a wet-wet format. Here are some quick numbers on flow rate over 2 hours, 3.3psi, including radial position:1x PBS (no protein)68 uL (r=4.1)91 uL (r=4.1)99 uL (r=4.5) Using Jim’s new equation. 99uL of…