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Cell Culture Applications
ByJimI’ve added a page on cell culture applications to the knowledge wiki. Watch this page and edit it if the topic is of interest to you. Remember the wikis provide a place to comment on the changes you make … and to compare and restore prior content … … so don’t be shy.
The Enemy Within: Optimizing an OLCN PUA Coated Microporous Membrane Mimetic and Exposing an Unlikely Saboteur
The battle for soft membrane coatings wages on as we continue to optimize polyurethane acrylate (PUA) coatings for our osteomyelitis project, whose background is described here and here and initial progress is described here. Since our last update, we have honed in on our most promising recipe and tried to fine tune its settings in…
10 dynes/cm^2 bottom channel Aline
AlineFlowBottomChannelSup AlineFlowBottomChannel
Pressurized Gold Separations
Motiviation and Background: Nakul and I have been performing a lot of DNA filtration and attempted DNA shearing experiments. We found that large DNA fragments (>1000 and >2000 bp) pass through ~30-40 nm max pore size membranes when pressurized to 10 psi (even in low salts). This is in contrast to the Bernhard data where…
Biofiltration – The Stirred Cell Edition
ByAndreaIntroduction: The lab purchased an Amicon 8010 Stirred Cell which has a 25mm diameter for membranes and holds 10mL of solution. The device works by placing the solution into the cell, pressurizing the system at 8-10 psi, and creates stirring via an elevated stir bar and the stir platform. This type of filtration system was chosen…
pinhole-less membranes
Inspired by JP, we decided to try to etch amorphous (unannealed) wafers this week to see if pinhole density changed. To my surprise, the amorphous membranes had no pinholes! Furthermore, I tried to anneal the membranes after the etch and they went flat indicating crystallization! Refer to the table below for the results. Wafer 565:…