Similar Posts
Diffusion tests with Nylon and Nitrocellulose
Last week I tried to diffuse Cytochrome C through our membranes onto PVDF and PES membranes to see if we can detect protein adsorption on the other membranes. The trial illustrated that cytochrome c will diffuse through our membranes and stick to the PVDF and PES membranes. I ran the experiment again, but I used…
Monolith Wetting with Ethanol Vapors
Summary I need to replace the function of my breath to get water into the femtoliter sized cavity of the monolithic device. I achieve this using a small stream of ethanol vapor as a precursor, and then replace the ethanol with water. Once the chips are wet, they should be reasonably stable until they can…
Bad to the Bone: Hydroxyapatite Coating Silicon Nanomembranes
In support of the bone infection studies done in the Schwarz lab at URMC, I have recently been tasked with coordinating a new collaboration with Dr. Yates lab in the Chemical Engineering department. The goal of this collaboration is to establish a method to coat our membranes with hydroxyapatite crystals. Two different methods were attempted,…
30 nm pnc-Si bias series
We have already established that bias is an important parameter in controlling the morphology of 15 nm thick pnc-Si, but have yet to look at its effect on thicker films. This week, made a few 30 nm thick Si films with 02, 05, 25, and 50 W of substrate bias. I annealed these films at…
Reviewing Simulation/Experimental Comparison
Here is the most up to date figure that I’ve made to compare the simulated and experimental separation results. I went back through the x-ray crystals and remeasured the longest distances in the molecules. I used the radii to rerun the simulations and plotted them against protein and rhodamine diffusion data. Here are the results:…
Reviewing membrane impact
To identify how much of an effect the membrane has on particular simulations, we originally came up with the idea of using a phenomenological effective diffusion to view the membrane impact (see this post). Here is the most up to date version of the figure, which was slotted for use in the simulated diffusion paper:…
Beautiful images of aligned cells on the backside of the device! Did you take any images before flow so we can make the claim these are aligned? You mentioned that some cells didn’t survive? Can you explain where?
I might have an image of these cells before flow but it is through the clamp system.I’ll make sure to get those before and after shots when I run this for the AFM measurements. I’ll add to this post next week. The cells that are in the channel of the aline are undergoing much higher shear stresses and the cells get strip off. I noticed that the entrance side seemed to retain more cells then the exit side but maybe it is just me. The membrane is 100% confluent and healthy and I can run two Alines with same system!