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Porosity vs. Cell Morphology
As I was preparing for the BMES conference, I took a few pictures of endothelial cells on pnc-Si transwells that showed extensive vacuole/endosome expression (here). The most dramatic example occurred on a 50nm-thick membrane that was barely porous. So I wondered – are cells responding to mechanical or porosity differences (or both, or something else)?…
Cell Suspension, Discoloration and Immunofluorescent assays – Marian
BymarianDiscoloration Assays Introduction: pnc-Si chips were monitored for discoloration to find chips that had not been previously RTP’d. These assays were necessary in order to find chips that could potentially be used for cell proliferation assays later. Setup: pnc-Si chips were autoclaved and placed in a 24-welled plate with 300uL of DMEM and incubated at…
Protein Adsorption on silanized NPN Chip
ByHenryWe are working with the Shestopalov group to silanize the nanoporous silicon nitride (NPN) membrane chip. In an ideal scenario, the silane would then be conjugated to PEG to ward off protein adsorption. In the presented work, we study protein adsorption on both the untreated and the silanized NPN chips using epifluorescence as the imaging modality….
Pore Processing Continuation
In my previous post, there still appears to be a discrepency between the calculated pore statistcis and the experimental permeabilities. I explored what I could do with the pore processing to bring the experimental closer to the calculated permeabilities. I set the minimum pore cutoff higher (15nm) and tried to remove any pores that did…
Visualizing increase in cut-off sharpness of carbonized alumina coated pnc-Si
In my last post, I introduced the concept of pore size dependent carbon growth on alumina coated membranes. Here, I have plotted a Gaussian fit of the tail of the pore size distribution of carbonized membranes (with and without an alumina coating). I am only showing the tail of the distribution to highlight the sharpness…