Negative Adsorption Assay Retry
This is a retrial of the previously outline negative adsorption assay. I was a little more careful with cuvettes this time around (there are very significant differences in the plastic cuvettes) and I spread out the same amount of solution over a larger area. If exposed to more surface, more protein should adsorb and leave the solution.
In the following figure I show the concentration of the remaining solution on three pieces of coverslip glass. The line is the standards curve (with an R2 value of .9998) and the points are the average of two measurements for each piece of glass. The dotted line is the concentration of the starting solution.
It seems that there is less error in this case, but the spread between the separate measurements is pretty large. This may make it difficult to compare to other materials.
I also tried to evaluate cellulose with this method, but the material soaked up the entire spread out sample. This method still needs to be tweaked a little.