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Desalting: Continued Work
In continuation of the desalting work, I ran three additional controls: a SepCon with no slits (negative control) and direct addition of 4M NaCl producing final dilutions of 1:400 and 1:600 (these are the experimental final dilutions I have been working with). The results are shown below. I also calculated the flux for each condition….
Fibroblast adhesion study using controls
Fibroblast adhesion experiment was done using poly-lysine coated plastic and teflon coverslips as positive and negative controls repectively. Poly-lysine is a polypeptide that gives the cells a sticky surface to adhere to. Teflon coverslips are used as negative control because they do not promote cell adhesion. Difference in morphology of cells could also be seen…
Drifting and Biasing Potentials in the Electrodes Make Streaming Potential Measurements Difficult
A few weeks back I had finished my streaming potential/electro-osmotic pump setup. It looks like this: For streaming potential measurements, we pressurize a tube filled with 100mM KCl that connects to the membrane and measure the voltage drop across the membrane. When I first applied a pressure to the setup, the voltage drop did seem…
hiPSC Differentiation to Endothelial Cell Culture Method, Brain Microvascular Endothelial Cells (EECM-BMEC-like cells), Round 2
Introduction This post highlights results from my second attempt to differentiate hiPSCs into endothelial progenitor cells EECM-BMEC-like cells in our lab. For details on methodology, see Nishihara et al., FASEB, 2020 and my previous post. Methods Cells were seeded at 70,000 and 100,000 cells/well on D-3. Only the 100,000 cell/well plate was sorted. Due to media being backordered,…
BBB co-culture (2)
In my last post (the first co-culture), I showed that bEnd3 cells exhibited a different morphology when they were co-cultured. I also decided that I would reverse the orientation of the co-culture in order to better visualize the endothelial barrier. That is, grow bEnd3 on the basolateral side and NG10815 on the apical side of…
Preliminary Fabrication of a Tenting Well Oxide with a Buried Electrode
We want to create a structure that can more easily control the electric field inside of the Monolith cavities, by adding an electrode specific to each well. My solution is to create a hybrid film stack, replacing some of the SiO2 spacer with a gold layer that is in fluidic contact on the walls of…
Can we export ppt files to html and upload those files with a link? I prefer not to have download anything to read anything on the blog.
but my animations won’t show! ok, i changed it to a .pdf