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Tensile Mechanical Testing of Ultrathin membranes
As part of Aim 1 of my PhD, I am looking into mechanical characterization of ultrathin membranes to explore the effects of porosity on the structural integrity and determine how porous we can go with a porous membrane, and last but not least, to quantify the mechanical robustness of Parylene membranes. Recently, the BME department…
Protein Adsorption on silanized NPN Chip
ByHenryWe are working with the Shestopalov group to silanize the nanoporous silicon nitride (NPN) membrane chip. In an ideal scenario, the silane would then be conjugated to PEG to ward off protein adsorption. In the presented work, we study protein adsorption on both the untreated and the silanized NPN chips using epifluorescence as the imaging modality….
SEM images for Micro & Nano Scale experiments
ByAslanHello Everyone, I have been trying to take SEM images in 3 different steps during my experiments with polystyrene beads over Track Etched membranes including before capturing, After capturing and releasing. Micro Scale Experiments: As you might remember, in Micro-scale experiments, we use 10 micron beads and 8 micron pores. Before capturing: This is only…
SiN NP wafer #'s 1029 and 1030 pore size revisions
Our work with permeance and hydraulic permeability tests on the SiN-NP seemed to show that the porosity and pore sizes I had calculated from the STEM images were too high. I used URnano’s TEM to get better quality images and, as suspected, the pore sizes need to be revised smaller. Wafer # 1030 (40 s…
Concurrent TEER and Phase Microscopy with Yuchen Wang
For the first time, we did time-lapse phase microscopy (1 frame / 5 seconds) looking at endothelial cells through the ITO in the static BBB device (device A bottom left well) and measuring the TEER concurrently. Here’s the video, just shy of 15 minutes of recording. (reporting a low resistance: 5-10 ohms, shorted). TEER Test…
Bead Aggregation Experiments (Beads + Protein)
Here are the first experiments I have performed with the SepCon slots, under the guidance of Jamie and Kilean. The goal of these experiments is to prove that goat anti-human beads (6.8 um diameter) can be combined with a low concentration of Human IgG (1 mg/mL) which will cause aggregation, and therefore accumulate on the…


At what pressure was the data in the table acquired?