The future of the Goergen Hall Basement

This post presents the data from all of my TEER experiments of BBB co-cultures, with bEnd3 endothelial cells on the bottom and NG101815 glia on the top of PET and pnc-Si transwells.  For all experiments, I used 50000 cells/cm2 as the seeding density.  I always had 3 samples – bEnd3 alone, NG10815 alone and the…

Today, I finally demonstrated liftoff of microporous SiOx from a bare Si wafer.  The sample is a 2 x 2 cm section of wafer 209A02, which has a 100 nm layer of TEOS SiOx that stress stabilizing anneal.  The film was patterned with close-packed ø3 µm pores (6 µm pitch) using S1813 resist, G-line stepper…

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A quick update since we last talked about how DNA controls were proving to be problematic: Things are working again (more or less). I went back top basics, remade most of my solution, ordered new DNA samples, and, most importantly, piranha-cleaned my chips. The first three experiments I did on basic 30-nm thick 5.4mm square…

I am having some problems with my electroosmosis device and I was hoping that people with more knowledge about the TEM chips might have some suggestions.  For these trials, I used membranes from a wafer labeled 412-S.  I am still using the PDMS device from my last post although now I have mini “clamps” that…

Just to give a brief background, I have been working with endothelial cells to make a microvessel mimetic device, and one of the important requirements for endothelial cell culture is to be able to grow the cells under physiological levels of shear stress. Further, since I am trying to achieve neutrophil transmigration through the endothelial…