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Changes in morphology… from oxide?
Looking back at production over the last year, I noticed that our porosity has dropped considerably. Now that we have a good handle on controlling pinhole density, it is time to focus on controlling morphology again. Below are two examples of a 15 nm and 30 nm pnc-Si film produced this month and one year…
Comparitive Functionalization of pnc-Si measured with f-BSA fluorescence intensity
The last of the larger windowless pnc-Si chips were sent to both Adarza and Dr. Shestopalov’s lab for functionalization. Adarza returned chips with PEG-1 and PEG-2 (different rounds of processing) with both 2 hours and 16 hour treatments. They also returned a set treated with an alcohol leaving group ,ethanolamine (EA). They did also supply…
Discoloration – Cell Media
I’ve been learning the discoloration protocol from Karl and Anant and finally have some pictures of my own to show. We got a couple of new media (RPMI and DMEM/F12) last week in order to grow the cells I’m getting from the Elder lab (rat lung cells). I did a discoloration test to see if…
Surface modification of PDMS for enhanced cell adhesion and proliferation
PLEASE read this journal article for NRG on Tuesday March 18th @ RIT (PDF links are at the bottom) Surface Chemical Modification of Poly(dimethylsiloxane) for the Enhanced Adhesion and Proliferation of Mesenchymal Stem Cells Shreyas Kuddannaya,†,§ Yon Jin Chuah,‡,§ Min Hui Adeline Lee,‡ Nishanth V. Menon,‡ Yuejun Kang,*,‡and Yilei Zhang*,† I’d like to discuss this paper…
Tomograms of 1148 NPN
Wafer 1148 was produced roughly around the same time that the wafer I used to make my oldest MgF2 material (wafer 1085) was produced. We know that some of the NPN material from that time was not etched all the way through, creating pores that look like a ‘bowl-with-hole’. I believe the shape of the…
Desalting BSA: Blank pnc-Si and Mini-Dialysis Units
The ultimate experimental goal of the desalting project is the removal of salts from a salt/protein solution while fractionating certain sized proteins with maximum protein retention and recovery.Thus far we have characterized the flux of salt through our membranes which show flux an order of magnitude greater than cellulose membranes. Our next step is to…