Utilization and development of in vitro µSiM platforms to study Bacterial Invasion of the Osteocyte Lacuno-Canalicular Network – MPS World Summit 2024 Poster

A few weeks ago, Josh and JP brought me some 3 window microslit membranes that had edge aligned 8 and 9 μm slits. While these may seem to be quite large for most applications, they are in fact perfect for working with whole blood. The experiments that we have planned for these membranes are based on the…

In this post, we’ll discuss a little bit about how would the deposition temperature affect the pore formation and pore morphology. I first show some previous results of the effect of deposition temperature on the porosity and pore size from Dave. From this plot, it shows that the relative low deposition temperature barely affects the…

This week, we explored the possible differences a thermal oxide making layer had versus a TEOS on membrane morphology. Here’s what we found: The top image shows wafer 410 @ (1,0) and the bottom wafer 503 @ (1,0). Both wafers were annealed, cleaned, sputtered using the same conditions. The only difference was the masking material….

Before starting my transport project with the use of the Tecan, it was important to see how the Tecan worked with the sepcon and transwell kept in the well plate. The goals from this experiment were to: 1. Test for fluorescence from the plastic. 2. See if fluorescence from the apical side has an affect…

So I got few nanonitride chips with a pncSi cap on it from Josh (#1019, 320 um sub, 50 nm SiN, 40/20). I annealed them before using it for cell culture. Instead of using the existing CytoVu designs, I made some household cell culturing platforms as shown in the figure below. filled the whole petri…