Similar Posts
Separation Model Update
In the past couple of weeks I’ve tried a few more molecules to flesh out the experimental verification of the separations model. This is a quick update of the results. In the following figure, the blue line is the predicted sieving curve, the green line sieving given a no membrane/free diffusion situation, and the blue…
ALine mod-µSiM: Version2
Modular µSiM Version 2 (V2) Holds ~30 µl of media in the bottom channel. Designed for cocultures, ELISAs, and other assays that are easier with greater channel media volumes. For more surface area and volume information on mod-µSiM V2, see ALine Modular Device Geometry. Passive Pumping Another feature of V2 devices is their ability to…
Preliminary Testing for Transwell Cancer Exosome Co-culture Studies
Last time this project was discussed at NRG, Henry was the project lead (we miss you, Henry). He was observing bladder epithelial cell (BdEC) invasion and migration in co-culture with T24 bladder cancer cells. Here are links to his previous posts. https://trace-bmps.org/bdec-t24-co-culture-wound-healing/ https://trace-bmps.org/bdec-t24-co-culture-invasion-assay-preliminary/ We have transitioned away from the invasion assay and moved towards transwell…
Kidney Week!
I went to Kidney Week in Philly, Nov 13-15. American Society of Nephrology. I spent most of my time in the Bioengineering talks and posters. Here are some highlights. I put the Abstracts pdf on the cloud. Recellularization of Vascular and Tubular Compartments of Rat Kidney Scaffolds with Embryonic Stem Cells Basically, they perfused…
Decreased Permeability with "dry" Microcons
ByMike HWhen I first starting testing the microcon filters I noticed that if they were allowed to sit overnight (exposed to air) after having been already tested with water the next day if they were tested again the permeability of the membrane decreased significantly. The membranes come pre-wet with trace amounts of glycerin; spinning with water…
W612 Pinhole Counts: Interesting Features
I am in the process of inspecting each membrane in the newly created wafer 612. First of all, some samples (~10% counted so far) have tears on the membrane edges, shown below. Images were taken at 20x microscope magnification (reflection image followed by transmission). I also have been seeing circular defects which do not penetrate…