Similar Posts
Shear-free chemotaxis: nanoparticle tracking showed significant flow reduction conferred by the membrane
ByHenryTo assess how shear-free our [ “shear-free” chemotaxis system] is, we tracked the motion of nanoparticles in the shear-free compartment as we input a known flow rate in the flow compartment. The nanoparticles we use are the 210 nm dragon green fluorescent beads. ==================================================================Following is a 6-min long movie of the nanoparticles flown at a flow…
Long Term Urea Clearance in 100% Serum
I ran a 30 hour urea clearance with 100% serum through the single slot dialysis device. The now standard protocol was run. The single slot chip was annealed in the RTP for robustness then clamped into the acrylic/PDMS flow fixture. Ethanol was pumped through the chip for 1 hour, then degassed PBS was pumped for…
EV Enrichment with Izon qEV Column
Introduction This post will describe the preliminary work I have completed using size exclusion chromatography (SEC) for EV enrichment. We are ultimately interested in interrogating serum EV biomarkers using CAD-LB, however, the presence of protein and lipoprotein contaminants reduces the capacity for EV capture (~104) on NPN membranes and may hinder the analysis of rare…
Control Experiments for NPN Translocation Project
Just a quick update this time – since my last post I have been doing control experiments to demonstrate the effect of the filters. For the controls I have been using the chips without the nitride cap, since I am running low on the nitride ones (current I have 23 left, 7 with filters already…
Dialysis Rotation Project
Introduction Throughout my rotation, I have been working on the dialysis project with Alec. The goal was to set up a multi-pass system to analyze the membrane’s ability to separate different molecules in blood. The critical separation is between β2-microglobulin (12 kDa) and albumin (66 kDa) – β2-microglobulin is a large toxin that needs to…
CAD-LB: Biomarker Detection Validation – ICAM-1 HUVEC Stimulation Model
Introduction This post is another update in my series of experiments aiming to both characterize and validate the CAD-LB platform. We have now shifted our focus to validating CAD-LB biomarker detection which will be done using multiple experimental models. The most recent model, and the topic of this post, uses HUVEC cells to validate the…