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Magnesium Fluoride Burst Pressure Wafer #2
I was not entirely satisfied with the amount of data that I received from my last burst pressure wafer, so I processed another wafer. This wafer had some yield loss initially, as well as window sizes that were slightly smaller than expected (by about 50 microns). We expect the mechanical strength of the windows to…
Rhodamine transport in transwells
I finished the first experiment to look at rhodamine transport across commercial transwell membranes. I was mainly interested if there would be enough transport into the basolateral chamber to measure the rhodamine with the spec. Experimental details: both chambers in PBS and at room temperature, spinning on the rotomix, 0.5mM initial rhodamine in donor; sample…
Designing a Reverse-Centrifugation Sepcon Variant (Part 1: Geometry)
We have an STTR grant funding us to revise the Sepcon plastic housing to enable us to do reverse centrifugation easier. As outlined in this previous post, we’re trying to remove unbound IgG from a solution of IgG-antibody conjugates using reverse centrifugation: The earlier work used Josh Winan’s jury-rigged PDMS-filled reverse centrifugation setup, but despite the…
Direct Capture and Analysis of Microplastic Particulate from Environmental Sources Using Silicon Nanomembranes: Some Highlights
Microplastic contamination in drinking water is a contemporary problem that has captured the public eye, and we’re now seeing a growing demand for scientific examination of the issue. In response to this, we have recently been working on establishing a microplastics capture and analysis platform with silicon nanomembranes for environmental water samples. We started by…
Preliminary Fabrication of a Tenting Well Oxide with a Buried Electrode
We want to create a structure that can more easily control the electric field inside of the Monolith cavities, by adding an electrode specific to each well. My solution is to create a hybrid film stack, replacing some of the SiO2 spacer with a gold layer that is in fluidic contact on the walls of…
Sepcon vs Cloning ring II
Repeated the previous experiment with W683 and following images were captured after 5 hours of incubation in DMEM(-FBS), Oven @ 37C. I took Sepcon samples with regular thin o-rings, fat o-ring, without o-ring and w/grease in place of o-rings. Also for comparison, used cloning rings and the 2D flat format (left=w/o grease, right=w/grease) samples. All…
Beautiful images of aligned cells on the backside of the device! Did you take any images before flow so we can make the claim these are aligned? You mentioned that some cells didn’t survive? Can you explain where?
I might have an image of these cells before flow but it is through the clamp system.I’ll make sure to get those before and after shots when I run this for the AFM measurements. I’ll add to this post next week. The cells that are in the channel of the aline are undergoing much higher shear stresses and the cells get strip off. I noticed that the entrance side seemed to retain more cells then the exit side but maybe it is just me. The membrane is 100% confluent and healthy and I can run two Alines with same system!