Modular assembly of the µSiM

EO tests were continued using 2 micron carboxylated PS Latex beads in a 0.2 mM K2HPO4 solution containing 0.1% tween. The solution had a pH of 7.54 and a conductivity of 67.0 uS/cm. The beads were negatively charged, and so would move towards the positive electrode absent electroosmotic flow. It was suggested that the pump…

Charles developed a new microEO chamber, and I did some quick particle tracking experiments.  The fluid in front of the membrane was filled with 200nm fluorescent polymer beads, and the power supply was used to cause the fluid to flow within the chamber.  Bead speed was measured with Henry’s particle tracking programs. Picture of microEO:…

I’ve been learning the discoloration protocol from Karl and Anant and finally have some pictures of my own to show. We got a couple of new media (RPMI and DMEM/F12) last week in order to grow the cells I’m getting from the Elder lab (rat lung cells). I did a discoloration test to see if…

A few weeks ago, professor Mike Aziz in Harvard University helped to annealed two samples and in-situ imaged the early stage of pore formation using their Jeol2010-hot-stage-TEM. The oxide seemed intact during the process. The sample was unannealed 20(bottom)/15(a-si)/10(top) three stack with removing the substrate. The first sample was heating to 693C. They took one…

Introduction Sphingosine-1-phosphate (S1P) is a bioactive sphingolipid with known paradoxical effects on endothelial cell (EC) barrier regulation. S1P binds 5 GPCRs: S1PR1 (Endothelial differentiation gene (EDG)-1), S1PR2 (EDG-5), S1PR3 (EDG-3), S1PR4 (EDG-6), and S1PR5 (EDG-8). Despite the aliases, endothelial cells predominantly express S1PR1&3. S1PR1 has been shown to have barrier protective or enhancing effects on…

This is a short review of a few experiments that Tom had set up this morning. Carbonized membranes: A few carbonized membranes that were used for permeability measurements were tested in EO.  The membranes were left in the sepcon, which was filled and placed in a reservoir.  Electrodes went inside the sepcon and the reservoir. …