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Scanning ion conductance microscopy of cells on TEM grids (pnc-Si, not annealed)
During the recent visit of Greg Madeijski to our Institute of Biophysics, Imaging & Optical Science (IBIOS) here in Nottingham we had the opportunity to try imaging cultures of cells on several nanoporous membranes, including CytoVu’s and also the unsupported TEM grids without SiN. Here is a short summary of our adventures, along with some…
8 hour diffusion
Because of the recent discoloration problems I observed with 24 hour diffusion using w670 and 673, I decided to try a shorter time point. 673 had a couple more samples to spare, so I tried an 8 hour diffusion experiment. No discoloration was observed: This is the normal color of the wafer. Slits were not…
Direct Capture and Analysis of Microplastic Particulate from Environmental Sources Using Silicon Nanomembranes: Some Highlights
Microplastic contamination in drinking water is a contemporary problem that has captured the public eye, and we’re now seeing a growing demand for scientific examination of the issue. In response to this, we have recently been working on establishing a microplastics capture and analysis platform with silicon nanomembranes for environmental water samples. We started by…
Testing CMFDA for Measuring Proliferation
ByAlanThe goal of the experiment was to use a fluorescent dye to label live cells in order to measure proliferation over several days. To avoid cell toxicity, the intensity of the fluorescence was reduced to ~1.5%. Human umbilical vein endothelial cells (HUVECs) were seeded in a a 96-well plate at a density of 5000 cells…
Discoloration – HBSS components
In my last discoloration experiment, I tested buffers with different pKa’s (MES, HEPES, and Tris). I found that only 10 mM HEPES in HBSS caused discoloration, and furthermore, that the HBSS component of this solution was the culprit (post). Fortunately, HBSS is a rather simple solution; it only consists of KCl, NaCl, Glucose, KH2PO4, Na2HPO4…
W509X Burst Pressure and Air Permeability
W509X, with single 100um by 1000um slits, was tested for air permeability and burst pressure. Prior to testing, I inspected all membranes for pinholes.; every sample had pinholes on the membrane perimeter, as shown below. The first two images are in reflection mode (pinholes appear black) and the last is a transmission image (pinholes appear…
Can you plot them from left to right with right being increasing distance from the center. Calculate x^2 + y^2 = R^2