Cell Spreading on pncSi and glass

  The initial purpose of exploring formlabs SLA 3D printer for a collaboration with Dr. Nicola Marchi (link to previous post). The purpose of the this work was gaining the ability to use a simple membrane on 3D printed platform for BBB modeling and WBC migration though endothelial (and glial cell layers) and collecting and…

For the last three weeks, we have increased the substrate bias power from 25 W to 32 W (for both oxide and a-Si) to adress some film quality issues.  I have found that it is “harder” to open pores in a film deposited at higher bias.  Below are micrographs showing the difference in porosity for…

I fixed the graph for the serial dilution of Rhodamine that I did last week and removed all absorbency values that were greater than 1.08 AU. The graph looks much more linear now with an R^2 value of .9517

Since the control experiments seem to be going OK I decided to try again with one of the NPN-tented structures that Greg made originally. Since piranha cleaning seemed to be making a difference I wanted to pre-clean the chip, but from previous experience with a couple of poorly-tented NPN chips as guinea pigs, the NPN…

I continued to run some of the samples that I described in this post last week.  These are P8 bEnd3 cells, seeded at 100000 cells/cm2 (just below confluence) and grown for 14 DAYS.  The pnc-Si was SC126 that was RTP’ed.  I measured the TEER of these samples for 2 weeks and then stained the cells…

In my last post I showed that using buffered solutions instead of Di-water we could obtained similar results with SC256 as we had with SC269. I used 20mM HEPES at pH7.5 with no salts and showed DDM diffusion through both “treated” and “untreated” SC256 samples. Additionally I showed that “treated” SC256 samples appeared to slow…