The purpose of this experiment was to quantify the effectiveness of the kit membrane at separating free antibody and qdots from qdot-bound antibodies. The manual advertises an approximate 80% yield for this step and no purity reading. It was originally planned to directly measure the purity based on the amount of free antibody in the…
Used Thermofisher SiteClick qdot antibody labeling kit 625. Began with 4.0mg/mL IgG and conjugated it with an unknown amount of qDots, methods provided by the SiteClick kit. The final stage in the manual [1] is an optional cleaning of the qDots to remove any unconjugated and free-floating qDots from the mixture using the nanosep omega…
Overview of Experiment This experiment examined the behavior of the sieving coefficient graph under mixed particle size conditions, where one particle was significantly greater than the average pore diameter, and therefore acted as a “plug” for the larger pores. With fewer undesirably large pores to let through larger particle sizes, the sieving curve steepens and…
