The relationship between the inlet and exit concentrations in a single pass over a membranes can be derived assuming first order loss to the membrane … (1) where is the local bulk concentration above the membrane and is the ‘elimination rate constant,’ a concept also used in drug delivery. this integrates across the membrane…
In my previous post, our urea clearance for the single pass experiments was found to be ~140 ml/min/m^2, which was similar to Fissell/Roy’s value except we used a flow rate of 1uL/min whereas they used a flow rate of 10 ml/min however. To show that we could achieve even greater clearance values than their group…
Microdialysis: Separation of BSA and Cyto C For the past few months I have been studying how well the 75 nm NPN-O membranes are at clearing cyto c while retaining BSA with and without the presence of PEG coating. Additionally, conductivity tests were performed to determine how well the membrane cleared a small molecule. See…
So in the past couple of months, I have focused on wrapping up the microdialysis project that was initiated by Alec. Just as a recap, this experiment utilizes a modified SepCon that features an open bottom to allow for separation of albumin and cytochrome c via diffusion. 50 uL of 1 mg/mL BSA and cytochrome…
Introduction Over the past two decades there has been little to no improvement in treatment for people who have end stage renal disease (ESRD). The two main methods of treatment for ESRD are kidney transplant or dialysis. However, due to the limitations involved with transplantation, the majority of patients receive dialysis. It has been speculated…
