Sartorius Internship Results – Last Aim of My Ph.D.
Hi all –
I finished my 10 months internship with Sartorius on 16th of July and since all my results are going to be the third and last aim of my Ph. D., I will be presenting my results in our NRG meeting. Since these results are confidential, I am not allowed to publish them anywhere, however, I have the permission to share and discuss them with you all. Please do not share these results with anyone out of our groups, Thank you.
Summary of the project:
Virus Counter 3100 (VC3100) is a flow cytometry-based technique for quantification of viruses. In this project, We showed that VC3100 can be used for precise quantification and characterization of extracellular vesicles (EVs). We compared the performance of VC3100 with NTA for precise quantification of nanoparticles in heterogeneous samples and found that VC3100 is superior compared to NTA. Furthermore, we showed that VC3100 is a powerful technique for quantification of individual particles and can be reliably used to count individual nanoparticles without concerns regarding coincidence or swarm detection of multiple nanoparticles. We created a method for quantification of fluorescently labeled EVs from different sources without any post labeling steps. This is very advantageous compared to fluorescent-based flow cytometry analysis of EVs, which always requires a washing step to remove the unbound dyes. By running control experiments suggested by a recent study published in the Journal of Extracellular Vesicles, we found that single vesicles can be successfully quantified by the VC3100. Moreover, we found that VC3100 can be successfully used for protein profiling of EVs against common exosome markers, tetraspanins (CD9, CD63, and CD81).
Thank You