Here are some more images from my ongoing cell culture project.
My previous work and protocols: ZO-1 staining of confluent RPE cells on various MgF2 substrates
I added twice the amount of primary antibody on these devices that I usually do (1:100 dilution, instead of 1:200).
I have been using MatLab to analyze the pores in the SC wafers. Here are the results from my analyses: To take a first look, I simply plotted four characteristics: average pore size, porosity, cut-off pore size, and mean roundness against the wafer number. Notice that the blue dots are the b wafers. Excel would…
Hi all, a quick update in case you want to know what we are up too. This summer it will be just me at the McGrath lab; the main goal is to produce some preliminary data with an eye on submitting an NSF grant in the fall. The topic is nano-biosensing, using Henry’s shear-free…
Membrane pore spacing can modulate endothelial cell- substrate and cell-cell interactions (REVISION) We submitted our “focal adhesions” paper to ACS Biomaterials Science and Engineering late last year. We received reasonable reviewer requests from three reviewers. I think I am mostly finished with revising the figures. Here are the updated figures and my comments on what changed….
Ag/AgCl electrodes are used overwhelmingly in streaming potential and sometimes in electroosmosis. The benefit of using Ag/AgCl in electroosmosis should be less gas generation and the ability to make an enclosed pump. Here is some information on my attempt at Ag/AgCl EO. .2mm silver wires were coated with AgCl using protocol. Electrodes were immersed in…
Here is an aggregate of the recent posts/data on the Hemodialysis Project … Device Design/Demo Design idea for assembling multiple chips Gasket design for counter flow dialysis chip Peclet chip demo – > Dialysis Membrane modifications to prevent protein binding (HD PEG) Protein binding is reduced by both Adarza and Shestopolov treatments Damage of pnc-Si by…
I oxidized 2 intact chips from w639 using the following protocol: 10min with secondary generator, 15min with both primary and secondary generators. This protocol is slightly different than my normal protocol (10min 2nd, 5min 1st+2nd, 10min 2nd) because I messed up the settings, although the current protocol is arbitrary anyhow since we haven’t tested it…
