FISHER team and PETL instructions

In the next paper that I’d like to publish, I will compare protein separations for two membranes with different pore distributions to a single membrane at two salt concentrations (basically a redo of this post).  My most recent trial with this had less than stellar results. I chose two wafers for movable cutoffs – SC…

Recently, SiMPore has been working on a new chip design which involves incomplete etches on both sides in order to create a fluidic system built directly into the silicon, consisting of many long, shallow trenches. These trench chips are designed to obviate the need for silicone gaskets on one side of the chip, reducing the…

As a quick update on the ECM masking with Parylene membrane, we made changes to the device designs. Instead of relying on the peel-off process, we used a simplified design, with the membrane serving as the roof of the channels. Here is the latest design:   For these experiments we continued to use relatively low…

  The NPN tenting mechanism I’ve described in a few different posts has relied solely on mechanical technique. I poke the substrate with tweezers, or delaminate it with high electrical forces, or heat it up and let it contract while in contact with each other.  It would scale very poorly if we tried to make this…

Here is the most up to date figure that I’ve made to compare the simulated and experimental separation results.  I went back through the x-ray crystals and remeasured the longest distances in the molecules.  I used the radii to rerun the simulations and plotted them against protein and rhodamine diffusion data.  Here are the results:…

I went over to RIT to work with JP on measuring the deflection of NPN and pnc-Si membranes as a function of pressure.  JP and Dave Fang did this work years ago, and we want to pick it up again to correlate with Sarah’s burst pressure data. We used the Veeco Wyko (white light interferometer)…